Journal: Journal of Bacteriology

Article Title: Corynebacterium diphtheriae : Identification and Characterization of a Channel-Forming Protein in the Cell Wall

doi: 10.1128/jb.00864-07

Figure Lengend Snippet: FIG. 4. Conductance (G) at a given membrane potential (Vm) di- vided by the conductance at 10 mV (G0) expressed as a function of the membrane potential. The symbols represent the means of four mea- surements, in which the 5-kDa protein from C. diphtheriae ATCC 11913 was added to the cis side of membranes. The solid line repre- sents the fit of the experimental data using equations 1 and 2 and the following parameters: n 1; and Vo 48 mV (left side of the curve) and Vo 46 mV (right side of the curve). The aqueous phase con- tained 1 M KCl, 10 mM Tris-HCl (pH 7.0), and 100 ng/ml porin. The membranes were formed from PC dissolved in n-decane. The temper- ature was 20°C.

Article Snippet: Comparison of the cell wall channel properties of C. diphtheriae, C. glutamicum, Mycobacterium chelonae, and Nocardia farcinica Cell wall porin Conductance (nS)in 1 M KCl Cation/anion permeability ratio in KCl Point charge at the channel mouth Channel diam (nm) estimated bya: Reference(s) or sourceLiposome swelling Single-channel conductance Effect of negative point charges C. diphtheriae ATCC 11913 2.25 1.26 None This study C. glutamicum ATCC 13032 PorA 5.5 8.1 2.0 2.2 2.2 38 PorH 2.5 5.1 2.0 2.2 2.2 30 PorB 0.70 0.12 1.5 1.4 18 Rhodococcus erythropolis 6.0 11.8 2.7 2 2 62

Techniques: Membrane

Journal: Journal of Bacteriology

Article Title: Corynebacterium diphtheriae : Identification and Characterization of a Channel-Forming Protein in the Cell Wall

doi: 10.1128/jb.00864-07

Figure Lengend Snippet: FIG. 5. Western blot analysis of CdporA of C. diphtheriae ATCC 11913. An SDS-PAGE gel containing CdPorA oligomers and CdporA monomers was blotted onto a nitrocellulose membrane, which was probed with anti-PorA polyclonal antibodies. Bands were detected using horseradish peroxidase-conjugated antibodies and chloronaph- thol-hydrogen peroxide as the substrate. Lane 1, C. diphtheriae porin oligomers; lane 2, CdporA monomers. Note that monomers and oligo- mers cannot be shown in one slot because of the very different blotting times of monomers and oligomers due to their different molecular masses.

Article Snippet: Comparison of the cell wall channel properties of C. diphtheriae, C. glutamicum, Mycobacterium chelonae, and Nocardia farcinica Cell wall porin Conductance (nS)in 1 M KCl Cation/anion permeability ratio in KCl Point charge at the channel mouth Channel diam (nm) estimated bya: Reference(s) or sourceLiposome swelling Single-channel conductance Effect of negative point charges C. diphtheriae ATCC 11913 2.25 1.26 None This study C. glutamicum ATCC 13032 PorA 5.5 8.1 2.0 2.2 2.2 38 PorH 2.5 5.1 2.0 2.2 2.2 30 PorB 0.70 0.12 1.5 1.4 18 Rhodococcus erythropolis 6.0 11.8 2.7 2 2 62

Techniques: Western Blot, SDS Page, Membrane

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 1. Native gel electrophoresis of the polyamidase from Nocardia farcinica after size exclusion chromatography (fraction 20 and 21). Protein bands were stained with silver. [Color figure can be seen in the online version of this article, available at www.interscience.wiley.com.]

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: Nucleic Acid Electrophoresis, Size-exclusion Chromatography, Staining

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 2. Sequence alignment of the 6-aminohexanoate-cyclic dimer hydrolase (EI) from Arthrobacter sp. KI72 (formerly Flavobacterium; BAA05090), the peptide amidase (Pam) from Stenotrophomonas maltophilia (CAC93616), with a putative amidase from Nocardia farcinica (YP_119043). The catalytic triad characteristic for amidase signature enzymes is framed, highly conserved regions are marked in gray and peptide fragments of the polyamidase from Nocardia farcinica (this study) matching with the putative amidase from Nocardia farcinica (YP_119043) are underlined. GenBank accession numbers are given in brackets.

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: Sequencing

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 3. Phylogenetic tree based on amino acid sequences of the polyamidase from Nocardia farcinica (fragments as identified by MS) and other highly homologous bacterial amidases as well as amidases with substrate specificity for 6-aminohexanoate oligomers. The alignment was created using the Clustal W algorithm (Thompson et al., 1994). Tree calculation was based on a sequence distance method and utilized the Neighbor Joining (NJ) algorithm (Saitou and Nei, 1987). Accession numbers given refer to GenBank while Arthrobacter sp. was formerly named Flavobacterium. [Color figure can be seen in the online version of this article, available at www.interscience.wiley.com.]

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: Sequencing

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 4. Increase of rising height of polyamide 6 fabrics after treatment with a polyamidase from N. farcinica compared to controls containing enzyme inhibitor and compared to the effect of silicone based finisher (‘‘chemical’’). Data represent the average of three measurements.

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: